FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469827
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC9080.19326262645612108No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA9060.19283693785159217No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA8010.17048828611382488No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC7680.163464424139098No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT7390.15729193937342895No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT6710.14281852681944632No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT6380.13579466484471944No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA6050.12877080286999257No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA5340.11365885740921658No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT5290.11259463589789433No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG5240.11153041438657209No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA5090.10833774985260533No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA4930.10493224101637412No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT8250.067.664884
TTCCGAT8600.066.6041642
CTTCCGA8850.064.7226941
CGATCTG5900.055.295155
CCCCCCG3200.045.3113029
TCCCCCC3300.043.9382328
CGATCTA2500.040.5989275
CTCCCCC3450.037.8250857
TCCGATC15950.036.8172073
GGGCGAC2501.8189894E-1234.799087
CTGGGCG850.002740867734.1167459
ATACTAA1102.4330501E-432.9571841
TTATATT2008.931238E-932.624144
CCTAGAC900.003626757832.221373
TAGTCTA900.003626757832.221374
ACTCCCC4300.030.3480346
ACCATAC5050.028.7151681
TACTCCC4900.028.1115045
ATACTCC4750.027.472964
CCATACT5350.027.1049732
Produced by FastQC (version 0.11.2)