FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280392
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5850.2086364803560729No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA5040.17974835230677053No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC4510.1608462438300665No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA4310.15371337270678193No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4070.14515392735884047No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT3960.141230848241034No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA3220.11483922508488116No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3200.11412593797255272No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3020.10770635396159661No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3020.10770635396159661No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT5350.062.345194
CTTCCGA5250.062.1737561
TATACGG350.003561847462.1515732
TTCCGAT5400.061.767922
CGATCTG2850.058.516985
GGGCGAC753.752375E-757.9977877
TCTTCCG3350.043.2896545
CTGGGCC1053.8105736E-641.434384
GTGCTCT4450.037.490541
CTAGCCT1001.3877507E-436.2550854
GTCGCGC1406.588998E-736.248628
TCCGATC9650.034.564433
CTATTCA850.002736753334.1224334
CTGGGGG850.002736753334.1224334
GGTCGCG1551.4604648E-632.7406857
CCTATAA4000.032.641221
AGGTCGC1801.2851888E-732.226746
ATTCTAT1153.1471497E-431.5374091
ACACATA1204.0515783E-430.207184145
TCGCGCG1703.0024294E-629.8518059
Produced by FastQC (version 0.11.2)