FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3c11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3c11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305848
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4900.16021030054144542No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC4580.14974758703669797No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA4370.14288143129920747No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT3930.1284952002301797No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3880.1268604012450629No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT3530.11541680834924538No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3290.1075697732206848No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA3240.105934974235568No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA7100.060.2549741
CCGATCT7100.060.245124
TTCCGAT7700.055.550692
CGATCTG4050.046.5421565
CGATCTA1807.094059E-1140.276875
GTATACA1651.3569661E-939.551031
TCCGATC10900.039.2422373
CCTTATA750.001480748238.672121
ATATAGC1001.3893764E-436.249183
CTACACA1402.7409187E-531.0707264
TACACGG950.004722739630.5256295
TATAGAG1952.597808E-729.7429182
GTCTAAT1255.141333E-429.0040871
ATAGCAA1753.764013E-628.9993485
CCTGGGG1000.006068623628.9993463
CGATCTC4400.028.0107335
TATACTG1050.007701071427.6184255
TACTCCG4100.026.5237925
TCTATAG1658.4011124E-526.3630432
GGCGACC1958.730085E-626.0250538
Produced by FastQC (version 0.11.2)