FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3c10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3c10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151494
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4450.29374100624447175No Hit
GATCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCG4040.2666772281410485No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3160.20858911904101815No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC2840.18746617027737073No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA2630.1736042351512271No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG2390.15776202357849156No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG2380.15710193142962758No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT2230.14720054919666786No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT2130.14059962770802806No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT1930.12739778473074842No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA1900.12541750828415646No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA1870.12343723183756451No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA1840.12145695539097258No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC1820.12013677109324462No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC1740.11485603390233277No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT1740.11485603390233277No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA1630.10759502026482896No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT1590.10495465166937305No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT1590.10495465166937305No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT1560.10297437522278109No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC1530.10099409877618916No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGC259.3468645E-487.057091
CCTAGGG502.2968525E-672.499672
CAGGGGC808.025381E-963.437214
GTTCGAA2700.061.75898145
CGCGGGG502.0048223E-457.9997373
CCTATAT400.00602535354.410681
CCTACAT400.006041125454.3747523
AGTGAGC400.006041125454.3747528
GTCGCGC953.115747E-853.420818
CCGTACT1405.456968E-1251.8196951
CGTACTA1405.456968E-1251.785482
CCGATCT2950.051.609944
TCGCGCG1004.6678906E-850.749779
CTTCCGA3050.049.9507831
GGGCGAC3950.049.556747
TTCCGAT3100.049.112682
ACCTAGG450.00959855448.365051
CCAGGGG752.540325E-548.333113
GCGGGGA752.540325E-548.333114
TCTATGG803.719668E-545.3122942
Produced by FastQC (version 0.11.2)