FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182193
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4560.2502840394526683No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC3880.21296098093779672No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA3620.19869039974093408No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3380.18551755555921468No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT2970.1630139467487774No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2940.16136734122606247No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA2610.14325468047619833No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG2610.14325468047619833No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG2390.1311795733096222No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA2310.1267886252490491No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT2280.12514201972633415No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT2270.12459315121876252No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT2170.1191044661430461No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC2080.11416464957490133No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT2050.11251804405218642No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC2050.11251804405218642No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC1990.10922483300675659No Hit
GATCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCG1980.10867596449918493No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA1910.10483388494618344No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT1890.10373614793104016No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGGG259.376032E-486.996049
CTTCCGA3950.077.127451
CCGATCT4050.075.181774
TTCCGAT4050.073.411882
CGATCTG2500.060.8972365
CGATCTA1102.5465852E-1159.3154875
TCCCCCC1405.456968E-1251.783368
CATACTA450.00961786748.344412
TACAGGG450.00961786748.344412
TACTGGG752.538861E-548.3444062
TCGCGCG1255.158654E-946.3978969
TCCGATC6750.046.195773
CAGGGGC951.9127838E-645.787394
CCCCCCG1602.0008883E-1145.310449
CTCCCCC1652.7284841E-1143.93747
ACTCCCC1703.8198777E-1142.6451236
GTACTGG700.001051753541.4494441
TGCATAG700.001054600541.426695
GTCGCGC1451.9137588E-839.998188
AGGTCGC1451.9137588E-839.998186
Produced by FastQC (version 0.11.2)