FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185242
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3930.21215491087334407No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA3590.19380054199371632No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC3420.18462335755390247No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3250.1754461731140886No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT3050.164649485537837No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2880.15547230109802312No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG2780.15007395730989734No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA2680.14467561352177152No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT2410.13010008529383185No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA2210.11930339771758025No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT2160.11660422582351734No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA2090.11282538517182927No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC2050.11066604765657896No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT1960.10580753824726574No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA1940.10472786948964058No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG1930.10418803511082801No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT1920.10364820073201543No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA1860.10040919445913994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT3800.068.701744
CTTCCGA4050.064.495721
TTCCGAT4200.062.158722
CGATCTA1400.062.1587145
GTATACG400.00602414354.4182621
GACACCC400.006043491454.374187145
TCCCCCC1004.675894E-850.7492458
ACACAGT450.00961713148.345676
CGATCTG2100.048.345675
CCCCCCG1056.866503E-848.3326159
TCTTCCG1851.8189894E-1243.1191025
ACTACCG700.00105308741.4391445
GTATATA907.423904E-540.3098261
CTGTGCA750.001480372338.6660929
TATATAC951.0241092E-438.1676333
AGTATAC951.0241092E-438.1676332
CATACTC1553.4364348E-837.4289053
TCCGATC7150.036.512813
CCATACT1604.544927E-836.2592472
CTCCCCC1406.5717177E-736.2494627
Produced by FastQC (version 0.11.2)