FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381629
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT98700.053.0372162
CTTCCGA99150.052.8830261
CCGATCT100200.051.374944
TCCGATC103900.050.522373
CGATCTG45200.048.924015
CGATCTA21500.035.4088255
CGATCTC37650.035.2408835
GATCTGG25650.029.679916
CGATCTT30100.029.6277925
CTATACT2951.4551915E-1129.4930634
GATCTCA14300.029.407136
CCTACAC4750.027.4751173
GTGCTCT67350.027.3505971
TCTTCCG68100.026.936065
TGCTCTT68350.026.731462
GATCTCG18400.026.0067926
GATCTGA17800.025.6614516
GTATATA6800.025.5960561
TCTGACC5500.025.0449498
GTGTTAA12000.024.7784021
Produced by FastQC (version 0.11.2)