FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423127
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC7800.18434181699584284No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA7410.1751247261460507No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC6340.14983681022482612No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5990.1415650620262947No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5660.13376598515339366No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT5180.12242187333826486No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT5160.1219492020126345No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG5020.11864050273322194No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA4910.11604081044225494No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT4870.1150954677909942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG4700.066.3297355
CCGATCT8250.065.030254
CTTCCGA9350.057.3864141
TTCCGAT9550.056.1779632
CTGTGCG700.001055953441.428549
TCCCCCC2700.040.2777448
CCCCCCG2750.039.545429
TAAGGGG750.001482424138.6666344
TCGCGCG2450.038.4693579
GTACAGT1553.4586265E-837.4237441
CATACTA1001.3900995E-436.2499732
ACTACCG800.002035297536.249975
TCCGATC15350.034.951113
CTCCCCC3150.034.523787
GTCGCGC2750.034.2726978
CCCTTAT850.002738629734.121651
GGGCGCT850.002740217134.117629
GGGCGAC2603.6379788E-1233.4615147
ATACTAC1801.2894088E-732.2221953
ATAAGGG1352.1393123E-532.2221953
Produced by FastQC (version 0.11.2)