FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640017
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA10990.17171418884185888No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC10670.16671432165083117No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC9770.15265219517606562No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT8310.12984030111700157No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA8180.12780910507064655No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT7940.12405920467737576No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA7850.12265299202989921No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT7760.12124677938242265No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT7460.11655940389083415No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG7040.10999707820261026No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA6460.10093481891887247No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA13350.070.0750961
TTCCGAT14450.065.237432
CCGATCT15150.063.1804354
CGATCTG9950.057.5738565
TCCGATC23300.040.4584053
CGATCTA3600.040.2855075
TCCCCCC3900.037.1779028
TACGGCA1001.391238E-436.248467
CCCCCCG4000.036.248469
CTCCCCC4600.033.096427
GTCGCGC2705.456968E-1232.220858
TCGCGCG2755.456968E-1231.6350179
GGTCGCG2901.2732926E-1129.9987247
ACTCCCC5200.029.2844646
GTGCTCT8700.029.1745431
TCTTCCG9100.027.8899655
ATACTCC6250.027.845344
TATAGGT4450.027.7019443
CGTGGGG2104.9733717E-727.6243483
TACTCCC6050.027.5672725
Produced by FastQC (version 0.11.2)