FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387873
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA5610.14463497072495377No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5330.13741611300606127No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC5130.13225978606399516No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4770.12297839756827622No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA4340.11189229464283411No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA3980.10261090614711516No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA7950.058.4019051
CCGATCT7950.058.3717884
TTCCGAT8500.054.594792
CGATCTG5050.047.3819245
TATAATA1453.8380676E-1045.00542
GTGCTCT4950.041.0361861
TCGCGCG2005.456968E-1239.874649
ATAATAC2450.038.4740073
TCCCCCC2650.038.3015488
CCCCCCG2700.037.592269
TTTATAC1553.457353E-837.4238433
GTCGCGC2002.237357E-1036.2496768
TCTTCCG5250.035.9090735
TCCGATC13550.034.7827723
GGGCGAC1707.802373E-834.1173447
CTCCCCC3050.033.2783937
GTATAAT1551.4546986E-632.762761
ACCATAC4300.032.055261
ACTCCCC3450.031.5255246
TTATACG1402.7401296E-531.0751574
Produced by FastQC (version 0.11.2)