FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494398
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC5670.11468492995521827No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5460.11043733995687685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA23350.052.181891
CCGATCT23200.051.8782924
TTCCGAT23750.050.9821852
CGATCTG10700.047.432925
TCCGATC27100.045.2150463
TCTAGGG1053.818519E-641.4312022
CTAGGGG1157.1322793E-637.828493
CGATCTC9100.034.2604185
CTCATAC1551.4622965E-632.7440153
ACTGTGG1402.7445956E-531.070268
CGATCTT7900.030.2867325
CGATCTA5350.028.4597515
GTGCTCT15500.027.1389921
GTATGCG1100.00964966426.3733141
CATACCG1100.009663952526.365313
GATCTGG5500.026.3653096
TACGGGG1100.00966871926.3626447
GTATTAG2559.4323696E-825.5976281
TACTGTG1701.0305232E-425.5872737
ATCTGCC2859.8734745E-925.4376377
Produced by FastQC (version 0.11.2)