FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3b11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3b11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387175
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC9070.23426099309097953No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA7140.18441273326015367No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT7000.18079679731387616No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT6770.17485633111642024No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6670.172273519726222No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC6450.1665913346677859No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5410.13973009620972426No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC5030.129915412926971No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA5010.12939885064893136No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG4850.1252663524246142No Hit
TCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGA4720.12190869761735651No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4690.12113385420029703No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT4650.12010072964421772No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG4460.1151933880028411No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG4370.11286885775166268No Hit
TCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGT4270.11028604636146445No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC4250.10976948408342481No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGAC4230.10925292180538515No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG4120.1064118292761671No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC4090.10563698585910763No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA4080.10537870472008783No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTT4080.10537870472008783No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAA3910.10098792535675083No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA12100.078.510341
TTCCGAT12650.075.0774462
CCGATCT13900.068.847454
CGATCTG6600.058.2184525
CGATCTC5550.052.2511335
TCCGATC18500.052.1205063
CGATCTA3350.051.939195
TCTTCCG5850.040.896565
TGCTCTT6700.036.790262
GTGCTCT6600.036.258591
TCTAGAC1001.3900497E-436.2492263
ATATACC1803.1905074E-936.249224
GGGCGAC3850.035.7784547
ATCTGAG2103.765308E-1034.5230757
GATCTCG2900.032.4993066
GGCGACC4250.032.411078
CTGTAAG900.003621301632.229861
CTAGGGG1352.1391459E-532.2215353
GATCTGA3750.030.9326736
GATCTAC2351.2569217E-930.8504076
Produced by FastQC (version 0.11.2)