FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3b10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3b10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206820
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4300.20791026012958128No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA3900.18856977081520163No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC3860.18663572188376365No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3620.17503142829513588No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT3390.16391064693936755No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT3250.15714147567933467No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA3020.14602069432356637No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG2880.1392515230635335No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT2700.13054830287206268No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA2540.1228121071463108No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC2510.12136157044773233No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT2410.11652644811913741No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT2240.10830674016052606No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT2230.10782322792766658No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT2210.10685620346194757No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA2190.10588917899622861No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG2130.10298810559907165No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA1600.063.4372865
CTTCCGA4750.059.5549161
TTCCGAT5000.056.549812
CCGATCT4900.056.22434
GGGCGAC2150.053.953317
AGGGGGC701.6913036E-551.785546
GTCTAAT604.9242424E-448.356561
GCGACCC2500.046.399849
CGATCTG2350.046.276445
GATCTAG1151.4053512E-744.1302876
GGCGACC2750.042.1816758
TCCGGAC750.001480688238.666543
ACGCAGG800.002032921936.2498786
CCGGACT800.002032921936.2498784
GGGGCGA3250.035.692196
GTATTGG1251.25610895E-534.816721
CATACTG850.002737027434.1175355
CCTACAC1501.1281572E-633.833223
TATAAGA1301.648506E-533.4614262
GTTCTAA900.003613118532.23771
Produced by FastQC (version 0.11.2)