FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458869
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC5970.130102491124918No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA25650.066.457611
TTCCGAT26800.063.8556632
CCGATCT26700.063.8162844
CGATCTG13050.063.338715
TCCGATC27750.061.924183
CGATCTA5250.049.7185065
TCTGTGC2150.043.8361558
TAGTGTG700.001056167941.4275747
TCTAGGC750.001482724138.6657378
TTAGTAC1001.3895816E-436.253083
GATCTGG9050.034.4504436
CCTAGTG850.002740769234.1168259
CGATCTC11300.034.0073135
GTGCTCT13250.033.9422041
GATCTAG2008.920324E-932.627776
TCTTCCG14000.032.109875
ATCTGTG3700.031.35067
ATCTAGG1652.3831617E-630.7568367
ATCTAGC950.004712632430.5422211
CTAAGAT1255.1469874E-429.0024644
Produced by FastQC (version 0.11.2)