FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294996
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6300.21356221779278364No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA5500.186443206009573No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC5260.17830750247460983No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5150.17457863835441836No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5010.1698328112923565No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4180.1416968365672755No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA4100.13898493538895443No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3540.12000162714070699No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3390.114916812431355No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA3190.10813705948555234No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA3170.10745908419097208No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT3070.10406920771807075No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3040.10305224477620036No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGT451.1925235E-464.443854
CTTCCGA5300.057.481531
CCGATCT5950.052.394474
CCTAGAC657.328479E-444.6149673
TTCCGAT6900.044.130022
CGATCTG3600.042.2912755
CCAGGGG700.001055477441.428183
GTATGCG750.001481757338.6663069
TAGGACA951.02718564E-438.1575364
GTCCAGG800.0020293136.268111
CTAGACC850.002738992734.117334
TCGCGCG1102.4321413E-432.9542359
CTAGACT900.003624282532.2219244
CACGAGC1153.153771E-431.52679145
GTATATA1204.039342E-430.2234211
GGTCGCG1204.051403E-430.208057
CGAGTCG2202.2530003E-829.6588136
GTCGCGC1255.145423E-428.999738
GTCGGGT2252.8032446E-828.999739
TCCGATC11150.027.9593813
Produced by FastQC (version 0.11.2)