FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896278
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG10120.11291139579460838No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC9660.10777905962212617No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC9440.10532446406137382No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT60350.059.5919842
CTTCCGA60650.059.5562631
CCGATCT62600.056.5234874
TCCGATC68000.052.7812583
CGATCTG30850.052.4122965
CGATCTC21500.038.108645
CGATCTA14200.036.253795
GATCTGG16300.034.696886
CGATCTT17850.033.715015
GATCTGA11550.032.016346
CCTATAG1153.1598998E-431.525043
GATCTAG6350.030.8299966
ATCTGGG7150.029.4053927
GTGCTCT39300.029.3449551
TATAGTG2303.4833647E-828.3725365
GATCTCG8650.026.8236166
ATACTGG1658.4116255E-526.3663946
TCTTCCG43200.026.351145
TGCTCTT43400.026.2297042
GCTCTTC44450.025.6101043
Produced by FastQC (version 0.11.2)