FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341407
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA7380.21616428485649095No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC7050.20649840220030635No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA6640.19448927526383467No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC6040.1769149431616809No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5740.16812777711060406No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT5380.15758317784931183No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA4880.14293790109751703No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT4290.12565647453039921No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA4010.11745511954939411No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3960.11599059187421465No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT3960.11599059187421465No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT3900.11423315866399927No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3790.11101119777860442No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA3780.11071829224356854No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA3690.10808214242824547No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT3620.10603180368299421No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC3440.10075950405234808No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCTA406.6532426E-572.508066
TTCCGAT6950.058.4237562
CCGATCT7150.056.7895244
CGATCTG3450.054.643755
CTTCCGA7600.052.511391
GTCCAGG604.9199554E-448.374131
CCTGGGC450.00962951348.3387033
ATACTAG1001.382649E-436.28061
CGATCTA1803.1832315E-936.254035
TACACCG1604.570029E-836.254035
GTAGCGC800.002035093336.248728
ACTACCG1655.9912054E-835.155425
GTGTAGC1251.2616922E-534.8038676
ACTAAGT850.002737976134.1214373
CTAACGT1551.4599973E-632.745573
GTATATA1801.2786586E-732.249421
GTCCATA1801.2786586E-732.249421
TCCGATC12900.030.9142883
AGAGGGC950.004720415530.5297095
GATCTGG3100.030.4066036
Produced by FastQC (version 0.11.2)