FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492658
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA20940.4250413065453113No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC17890.36313223371994363No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC16440.33370005155706395No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA15600.31664968395925774No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT15160.3077185390270736No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT13790.27991020139731826No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT12950.26285983379951205No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA12010.24377966053530034No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG11010.22348160387124535No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA10510.21333257553921786No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA10460.21231767270601512No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT10100.20501037230695532No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA9990.20277758607390928No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT9130.185321257342822No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT8950.18166760714329208No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA8860.17984078204352716No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG8660.17578117071071617No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT8560.17375136504431066No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC8250.16745896747845362No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC8250.16745896747845362No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA8030.1629933950123615No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG7950.16136955047923712No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG7920.16076060877931547No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA7630.15487417234673953No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT7460.1514235027138502No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC7300.14817581364760138No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG7270.14756687194767973No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT6930.14066553268190105No Hit
CCTATGATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGA6800.13802678531557389No Hit
TAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCTTTGGTGTGCATTGGT6640.13477909624932508No Hit
GTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCG6540.1327492905829196No Hit
GTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC6410.13011054321659243No Hit
CAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAGAGC6410.13011054321659243No Hit
GCCCCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACA6390.12970458208331134No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5970.12117939828440824No Hit
CCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT5940.12057045658448659No Hit
TCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTC5890.11955555375128385No Hit
ATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGG5650.11468402015191066No Hit
ATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGT5640.11448103958527012No Hit
CTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAA5540.11245123391886462No Hit
ATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAG5530.11224825335222406No Hit
GTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTC5130.10412903068660206No Hit
GCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGATAACATCATA5120.10392605011996152No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC5030.10209922502019655No Hit
TCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGT4940.10027239992043162No Hit
GTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCTTT4940.10027239992043162No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT11250.085.7085342
CTTCCGA11300.085.329291
CCGATCT11850.080.145264
CGATCTG6200.071.32855
TCTTCCG5150.060.532165
CGATCTC4900.057.7023475
ATCTGCG1109.947871E-846.1349757
CGATCTA2450.044.386425
TGCTCTT7450.041.844382
CGATCTT3000.041.0820965
ACCATAC6150.040.0800971
TCCGATC24250.039.761693
GATCTCG2400.039.2696536
GTGCTCT8400.038.8381161
CCATACT6350.038.817732
GCCTATG1901.2732926E-1038.16837145
ATAAGCC1157.1368104E-637.824953
ATACTCC6800.037.3150524
ATAGTAC1209.5559735E-636.248913
TCCCCCC6850.035.9843188
Produced by FastQC (version 0.11.2)