FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339945
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5650.1662033564253041No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC5590.16443836502963716No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA5300.15590757328391358No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4630.136198502698966No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA4530.13325685037285442No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4530.13325685037285442No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA4070.11972524967274117No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3690.10854697083351719No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT3660.10766447513568371No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3480.10236950094868287No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT7550.071.058164
CTTCCGA7550.070.108231
TTCCGAT8250.064.1502152
CGATCTG5100.061.1262135
TCCGATC12050.045.1235663
GGGCGAC1252.712295E-740.5991747
TCCCCCC2057.2759576E-1238.9016538
CCCCCCG2109.094947E-1237.9754189
TAGACTG1209.5466185E-636.2492645
ACAGTGC1051.8513703E-434.523118
CTAGGGA850.00273972534.116953
TATAGAT1301.651812E-533.4608572
CAGTGCA1102.4330744E-432.9538769
TACACCG1352.1383665E-532.221575
TATAGAC900.003625249532.2215653
CTCCCCC2506.91216E-1131.8993517
GTATAGA1851.636381E-731.3553261
ATAGTAT950.004723422730.5256963
GGGGCGA1902.0701191E-730.5256966
CGATCTA2151.8033461E-830.3482235
Produced by FastQC (version 0.11.2)