FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329826
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5950.18039814932722104No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA5280.16008440814247513No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC5080.154020604803745No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5050.1531110343029355No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4510.13673876528836418No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT3910.11854735527217382No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA3750.11369631260118973No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3680.11157398143263417No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3430.10399422725922154No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3340.10126551575679298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT7450.069.093892
CTTCCGA7600.067.7404561
CCGATCT7850.065.573184
CGATCTG4600.064.61955
TAGACCC657.3294435E-444.6153375
TCCGATC12000.042.895793
CTACCCT700.001055615841.4285284
ATACCCG1451.9212166E-839.9999585
ACCTATA1209.534577E-636.255461
TCTTCCG4350.034.9999665
CCCCCCG1905.4023985E-934.3420689
TATTAGG850.002739348734.117612
CCGTGGG1301.6514463E-533.4615029
CTGGGGG900.003624751732.222193
GTGCTCT5000.031.9048061
CATATAC1601.8720912E-631.7187183
ACTACCG1153.157559E-431.5217085
TAGGGGA1153.157559E-431.5217084
ATACTAC1402.7411634E-531.0713963
TATACTG1402.7411634E-531.0713965
Produced by FastQC (version 0.11.2)