FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3a12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3a12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331989
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC7280.21928437387985747No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5690.17139122079346003No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA5210.15693291042775515No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4820.14518553325561992No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA4340.13072722288991503No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA4310.12982357849205847No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT4300.12952236369277295No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC4170.1256065713020612No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT4140.12470292690420466No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA3820.11506405332706807No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA3750.11295554973206944No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC3740.11265433493278393No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC3740.11265433493278393No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA3610.10873854254207219No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC3460.1042203205527894No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT3460.1042203205527894No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT7450.072.023754
TTCCGAT7500.070.576792
CTTCCGA7750.068.31041
GTCGCGC1600.058.905828
TCGCGCG1900.049.6049049
TATAGGT2050.049.5192953
CGATCTG4400.047.7909435
GGTCGCG2000.047.124667
GCAGGGG1255.171387E-946.4066543
TCTTCCG4650.045.2215355
GTGCTCT5200.044.6285061
CGATCTA2450.041.4345135
AGGTCGC2300.040.984146
CCGTGGG1105.2539326E-639.545179
TCCGATC13650.039.3096663
GGGGTGC750.001480913638.672216
CGATCTC3900.037.1848145
TATACAA1209.535233E-636.25525
CATACTA800.002033227636.2551962
AGGGGTC800.002033227636.2551965
Produced by FastQC (version 0.11.2)