FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3a11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3a11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255952
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC9110.35592611114583983No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA8250.32232606113646306No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC7940.31021441520285054No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT7700.30083765706069887No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA7610.297321372757392No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC6230.24340501344002No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT6210.24262361692817402No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT6110.2387166343689442No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA6050.23637244483340628No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT5490.21449334250171906No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT5100.19925611052072265No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT5080.19847471400887667No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA5060.1976933174970307No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA4990.1949584297055698No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC4750.18558167156341815No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA4690.18323748202788023No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT4630.18089329249234232No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT4530.17698630993311246No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG4500.1758142151653435No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG4460.17425142214165157No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC4340.16956304307057574No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA4270.16682815527911482No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT4210.1644839657435769No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC4110.16057698318434704No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA4020.1570606988810402No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG3950.1543258110895793No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA3760.14690254422704258No Hit
CCTATGATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGA3580.13986997562042883No Hit
GTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC3560.13908857910858288No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAA3490.13635369131712197No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG3460.13518159654935302No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC3430.13400950178158405No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGAC3370.13166531224604613No Hit
TCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGA3250.1269769331749703No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA3100.12111645933612551No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG3070.11994436456835658No Hit
GTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCG3060.1195536663124336No Hit
CAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAGAGC3010.11760017503281867No Hit
GCCCCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACA2920.11408389072951179No Hit
TAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCTTTGGTGTGCATTGGT2910.1136931924735888No Hit
ATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCA2820.11017690817028193No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT2780.10861411514658999No Hit
TCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGT2770.108223416890667No Hit
TAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCT2740.10705132212289804No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC2720.10626992561105207No Hit
CCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT2700.1054885290992061No Hit
ATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAG2680.10470713258736013No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG2660.10392573607551416No Hit
TTTCAAGACGGGTCGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGC2660.10392573607551416No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2630.1027536413077452No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG2580.10080015002813028No Hit
CTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAA2570.1004094517722073No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA6150.094.327251
TTCCGAT6250.092.799872
CCGATCT6650.089.398384
GTTAAAT259.371484E-487.0168841
CGATCTG3100.081.854735
CGATCTA1850.074.459365
ATCTGCG554.053016E-665.9090047
TCTTCCG2150.057.3255045
CGATCTC2500.055.0999225
CATAGGT400.006045795554.3749282
ATCTGGG1405.456968E-1251.785647
GATCTGG2150.050.581336
GGTTATA450.00962362648.3427121
TTAGGGG450.00963105948.3332673
TATGGGG450.00963105948.3332672
GATCTAC1203.603418E-948.3332676
CTGGGCG752.5452417E-548.3332679
TATAAAT604.9381744E-448.3332672
TCCGATC12850.046.264533
CTTGGGG657.326364E-444.6153263
Produced by FastQC (version 0.11.2)