FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2x7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2x7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34129
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG1260.36918749450613847No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC1030.30179612646136716No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC840.24612499633742566No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA700.20510416361452138No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT680.19924404465410647No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA640.1875238067332767No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC620.1816636877728618No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC620.1816636877728618No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT540.1582232119312022No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT520.1523630929707873No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT480.14064285504995752No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC460.1347827360895426No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG440.12892261712912773No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG430.12599255764892028No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT420.12306249816871283No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC400.11720237920829792No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT390.11427231972809047No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATT390.11427231972809047No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA370.10841220076767558No Hit
GCCCCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACA370.10841220076767558No Hit
ACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCG370.10841220076767558No Hit
GTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGA360.10548214128746813No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC360.10548214128746813No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT350.10255208180726069No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG350.10255208180726069No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAAGA100.007031392145.20413
CCGTTGC100.0070623513144.99127
GCAACAG203.8346896E-4108.74348
CCGATCT451.1973443E-680.668954
TCGCGCG300.00192015472.49569
CGAGTCG553.9449733E-666.001876
CTTCCGA553.9449733E-666.001871
TTCCGAT553.9449733E-666.001872
GTAGACA553.9449733E-666.001874
TACGAGT553.9449733E-666.001874
CGTAGAC553.9449733E-666.001873
GAGTCGG553.9796087E-665.90517
TCTGAAG350.0035173462.230333
GTCTGGG350.0035173462.230332
GTCGCGC350.00353789162.1390848
GGTCGCG350.00353789162.1390847
TAGACAG606.6105513E-660.5017135
GTCTACG606.6105513E-660.5017131
ACGAGTC606.6105513E-660.5017135
GACAGCG606.6685316E-660.4130027
Produced by FastQC (version 0.11.2)