FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2x6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2x6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331894
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4080.12293081526029395No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG4050.12202691220690944No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC3840.11569959083321785No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA3410.10274364706803979No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA5300.050.6268351
CCGATCT5650.046.2001654
TTCCGAT6300.042.584412
GTGTACG700.001054222341.4397241
CCCCTAG750.001479997338.6770741
CATAATA750.00148109438.6712464
TATACAT1354.9534356E-737.5970465
CTATACA1251.26152245E-534.8041234
TACTATA1352.136186E-532.226042
TTAGGTA950.004720057430.529934
CATATAG950.004720057430.529932
TGTACTG1204.0494508E-430.211915
TCTTCCG3450.029.4237755
TCCTAGG1255.1429437E-429.0034372
ACTATAC1504.3864176E-529.0034353
ATATAGC1504.3864176E-529.0034353
CGACTCA1000.006065176329.0034356
TCCGATC10050.028.1376613
TATAATT1306.469269E-427.8879182
CTATTAG1050.007691039727.6264821
Produced by FastQC (version 0.11.2)