FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2x5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2x5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474447
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC7520.15850031721140612No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG6720.1416385813378523No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA6230.13131076811530054No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC6140.12941382282952574No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5830.12287990017852363No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5640.11887523790855459No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGG753.7520113E-758.0110473
TATATAA858.90308E-751.186222
TTCCGAT8200.049.5216262
CTTCCGA8150.048.946021
CCGATCT8400.048.342544
GCCTAGA951.9134386E-645.8078541
CGATCTG5000.040.6077355
TAGTACA800.00203363636.2569054
GTAAGAC900.003622942432.228363
ACTACCG1153.156084E-431.5277445
GTGCTCT6350.029.6969551
GTATATA1504.3818814E-529.0116421
CGATCTA2502.4356268E-929.0055245
GTACATA1050.007689357727.6301351
ACATACC1358.067435E-426.8569683
CCCCCCG3255.0931703E-1126.7686869
TCCGATC15350.025.9821453
GACAGCG4800.025.6765617
GGGCGAC2559.466021E-825.5877157
TAAGGTG4550.025.4993635
Produced by FastQC (version 0.11.2)