FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2x4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2x4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138696
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC2160.15573628655476726No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC2050.1478052719617004No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT1980.14275826267520333No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA1930.13915325604199114No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC1870.13482724808213647No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA1470.10598719501643883No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT1390.1002191844032993No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCCG350.003555154262.1643455
TGCACTG350.003555154262.1643455
CTGTACG400.006040354.3741849
TTCCGAT2250.051.5733832
CCGATCT2350.049.3787734
CTGCGGA604.9218535E-448.3500482
AGCGTGT450.00962233648.332618
CCTCGCC450.00962233648.332619
CTTCCGA2300.047.298961
CGATCTG2100.041.4428985
AGGTGCC1157.0990664E-637.8255237
GACAGCG1604.5416527E-836.249467
CCCTGCA850.002729251534.1294482
ATAAGGT1301.6406744E-533.473114
CTATGGA900.00361142132.2333642
GGGCTGC900.00361142132.2333646
CATAAGG1352.1239717E-532.2333643
TAAGGTG1352.1239717E-532.2333645
GTGCTCT1601.8567152E-631.7297171
CGTAGAC1601.8567152E-631.7297173
Produced by FastQC (version 0.11.2)