FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2x3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2x3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322822
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG6750.20909355620125025No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT4670.14466176406812423No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTACT400.00604363754.3830761
CTTCCGA24750.050.6842961
TTCCGAT25000.049.8874052
CGATCTG12450.049.5053445
CCGATCT24850.049.0213624
TCCGATC26100.047.7848743
CGATCTA3600.044.3121345
GTCCTAG700.001054805941.4347231
TAAGCCG951.0264461E-438.1635634
GATCTAG2109.094947E-1237.981836
TAAGACC1251.2611568E-534.805174
TAAACTG850.00273725434.1227155
TATTAGC850.00273725434.1227152
ATCTAGG850.00273933434.1174287
GATCTGC8050.033.3279276
TTAATAC1352.1355681E-532.227013
CGATCTC8400.031.9392645
TATGTTG1153.1546433E-431.5264195
TTATAGT1402.738184E-531.0760424
GTACAGG1652.377068E-630.7621441
Produced by FastQC (version 0.11.2)