FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2x2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2x2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245739
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC8190.33328043167751153No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA6540.26613602236519235No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG6460.2628805358530799No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT6050.24619616747850365No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC5990.24375455259441928No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5890.23968519445427872No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG4980.20265403537899968No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT4660.1896320893305499No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT4610.1875974102604796No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4570.18596966700442338No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC4530.18434192374836716No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC4280.17416852839801578No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA4160.16928529862984712No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT4100.16684368374576278No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3930.15992577490752385No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA3730.15178705862724273No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC3670.1493454437431584No Hit
GCCCCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACA3630.14771770048710217No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT3590.14608995723104595No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT3540.14405527816097566No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC3440.13998592002083513No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA3430.13957898420682108No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT3420.139172048392807No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC3310.1346957544386524No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3290.13388188281062427No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG3240.131847203740554No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGAC3130.1273709097863994No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA3050.12411542327428694No Hit
TAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCT2860.1163836428080199No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG2790.11353509210992149No Hit
ACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACTCC2750.11190734885386529No Hit
CTCCTGGTCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAG2660.10824492652773879No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA2630.1070241190856966No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAA2610.10621024745766851No Hit
GTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATG2570.10458250420161228No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT2570.10458250420161228No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG2540.1033616967595701No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG2520.10254782513154201No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAT259.3652966E-487.0303145
TTCCGAT3900.074.369732
TATATAA406.648431E-572.510482
CCGATCT4400.065.9052054
CTTCCGA4300.065.778711
CGATCTG2900.064.996175
ATATAAC502.0058129E-458.0083853
ACCATAC2650.051.9992331
ACTACCG1004.6858986E-850.7470055
CCATACT2650.049.2524032
CCAGGGG604.9342314E-448.3403243
CGTAGCG604.9342314E-448.3403242
CATACTA1056.870141E-848.340322
ACCGTGG1203.603418E-948.3304828
CATACTC2800.046.6138843
CAGGGGC803.72768E-545.3098264
ATTAATA657.313183E-444.63092145
GTATATA657.313183E-444.630921
CCCAGGG657.3205226E-444.6218342
TCCCCCC2950.044.234688
Produced by FastQC (version 0.11.2)