FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2x12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2x12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670022
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC15470.23088794099298235No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG13930.20790362107512886No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA11830.17656136664169236No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC11280.1683526809567447No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT9930.14820408882096409No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA9380.13999540313601644No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC9310.13895066132156855No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT8610.1285032431770897No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC8420.1256675153950169No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT8000.11939906450832959No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT7380.11014563700893402No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA7310.10910089519448615No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT7130.10641441624304875No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT6920.10328019079970509No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT6910.10313094196906968No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG6830.10193695132398638No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA9600.064.2227251
TTCCGAT10550.057.7348672
CCGATCT11550.052.7283024
GGGCGAC5500.043.4976047
CGATCTG7150.041.5742385
GGCGACC7100.036.7585378
TCGCGCG3050.035.653779
CTAGGGG1854.1763997E-935.270963
CAGGGGC2154.8567017E-1033.721594
GTCGCGC3250.033.4596988
ATACTAG1551.4587131E-632.7572441
GGGGCGA7500.031.9006236
CCCCCCG5050.031.582429
TCCCCCC5100.031.2727878
GCGACCC7900.031.2008159
ACTCCCC5850.030.9835116
GTATATA2351.2551027E-930.865491
TCCGATC20300.030.7148883
TATAGGT3800.030.526913
ACCATAC6600.029.6729581
Produced by FastQC (version 0.11.2)