FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2x11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2x11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516195
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC10620.2057362043413826No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC9290.17997074748883657No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA8560.1658288050058602No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA8410.16292292641346778No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT8390.16253547593448212No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG8080.1565299935102045No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT6650.12882728426273019No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC6040.11701004465366771No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT6030.11681631941417488No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC5760.11158573794786852No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT5720.11081083698989723No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA5650.10945476031344745No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT5630.10906730983446178No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT5520.10693633220004069No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA5430.10519280504460524No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT5410.10480535456561957No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT8200.067.1950764
CTTCCGA8750.065.463441
TTCCGAT8600.064.912752
CGATCTG5350.063.691555
TAGGGCC350.003565535462.142824
CTATAGG606.797067E-660.4224781
TCCCCCC3650.043.69868
CGATCTA1903.6379788E-1241.9736565
CTCCCCC3900.040.8974087
GGGCGAC4100.038.9024127
CCCCCCG4150.038.4337129
CCATACT4400.036.2499732
GACGCTA1251.26391005E-534.799976145
ACCATAC4850.034.384751
ACTCCCC4650.034.3010526
AGGGGGC850.002740771534.1176265
GTATAAG1501.1316279E-633.836591
GTATATA1956.9721864E-933.4647561
TCCGATC16550.033.293033
ATACTCC5100.032.6960564
Produced by FastQC (version 0.11.2)