FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2x10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2x10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493383
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC17970.3642200886532369No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG16750.3394928483551318No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC14510.2940920137094306No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA14100.28578203951088704No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA13520.27402646625441085No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC13320.26997282030390185No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT12480.2529475073117639No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT11230.22761222012108243No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG10160.20592521428585905No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC10040.20349302671555364No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT10040.20349302671555364No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT10020.20308766212050272No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT9300.1884945366986702No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT9260.1876838075085684No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC8640.1751175050619904No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA8610.17450945816941404No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG8440.17106385911148134No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC8430.1708611768139559No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT8400.17025312992137953No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA8150.16518607248324324No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT8130.16478070788819235No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC8020.16255120261541237No Hit
GCCCCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACA7910.16032169734263238No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA6810.13802664461483272No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG6770.13721591542473088No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGAC6600.1337703163667982No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA6570.13316226947422186No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA6520.1321488579865946No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATT6250.1266764359534074No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAA6180.12525765987072923No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA6160.12485229527567834No Hit
ACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCG6160.12485229527567834No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5970.12100133162269475No Hit
ATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCA5960.12079864932516929No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG5890.11937987324249114No Hit
TAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCT5770.1169476856721857No Hit
GTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCG5720.11593427418455844No Hit
ACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACTCC5720.11593427418455844No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG5690.11532622729198208No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG5460.11066453444889669No Hit
GTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATG5320.10782698228354037No Hit
CTCCTGGTCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAG5300.10742161768848947No Hit
CTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAA5250.10640820620086221No Hit
TAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCTTTGGTGTGCATTGGT5050.10235456025035317No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC4980.100935784167675No Hit
GTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGC4960.1005304195726241No Hit
ACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAA4950.10032773727509867No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT8350.088.562622
CCGATCT9250.079.161944
CTTCCGA9850.075.106381
CGATCTG5650.073.141395
ACACATA1750.057.999836145
ACCATAC6700.048.7136421
TAGATAG450.00963637148.33325
ATACTCC7150.047.657214
CCATACT7000.047.6427232
TCCCCCC7200.046.3193138
GTATAAT951.9135769E-645.8079151
GTCTAAC1453.8198777E-1045.018121
CATACTC7450.044.7649773
TATAGGT4100.044.2071953
TCTTCCG3650.043.698515
TCTATAC1002.7313654E-643.4998783
TCGCGCG4200.043.154649
CGATCTC3550.042.8872035
CCCCCCG7850.042.483969
GTCGCGC4300.042.1510438
Produced by FastQC (version 0.11.2)