FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2h9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2h9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences801673
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG34580.43134794361291945No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC33600.41912350796397035No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG26200.32681654490047685No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC21790.2718065844802058No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC18030.22490466811280907No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC17800.2220356679094843No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA17640.22003984168108442No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT16480.20557010152518546No Hit
GATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAG16180.20182792734693575No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT15560.19409410071188626No Hit
ATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGCGCGAACTTC15340.19134983964783647No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG15290.1907261439514615No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC15220.18985296997653656No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC15190.1894787525587116No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA15170.18922927428016162No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT14770.18423970870916198No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG14210.17725431690976248No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT13820.1723894904780378No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG13780.17189053392093784No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT13660.17039366424963795No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG13210.16478040298226335No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG13050.16278457675386349No Hit
GGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGC12440.15517548925808902No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA12300.15342914130823915No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC12110.1510590976620143No Hit
ACCTCCGGCTGAGCCGTTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGA11940.14893853229433945No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA11820.14744166262303957No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA11720.14619427123028966No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT11690.14582005381246468No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT11530.14382422758406482No Hit
GTCTGATAGCGCTTAAGCGCGAACTTCGAAAGGGGATCCGGTTAAAATTC11110.1385851837345152No Hit
AGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGC11010.13733779234176527No Hit
GCACATGGGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCG10760.1342193138598905No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT10660.1329719224671406No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGT10630.13259770504931562No Hit
ACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTT10480.13072661796019075No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCG10460.1304771396816408No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA10370.12935448742816585No Hit
ATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAAC10350.12910500914961587No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTG10300.12848131345324093No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTT10080.1257370523891911No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAG9900.12349174788224127No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG9820.12249383476804133No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGAC9760.12174539993239139No Hit
TCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTT9610.11987431284326652No Hit
ATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGG9590.11962483456471654No Hit
CTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAA9480.11825270403269164No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC9420.11750426919704168No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA9320.11625687780429178No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAA9120.11376209501879195No Hit
GTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCCGGA9030.11263944276531702No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC9010.11238996448676705No Hit
GGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAAGAGTCG8990.11214048620821707No Hit
GATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGT8840.1102693991190922No Hit
CTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTT8830.11014465997981722No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT8660.10802409461214235No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC8640.10777461633359237No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA8620.10752513805504241No Hit
ATTTTAACCGGATCCCCTTTCGAAGTTCGCGCTTAAGCGCTATCAGACGG8590.10715092063721741No Hit
TTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCC8310.10365822473751767No Hit
GTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTT8220.10253557248404274No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCT8210.10241083334476776No Hit
CCCTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGT8190.10216135506621778No Hit
ATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAATGCCTCTAA8190.10216135506621778No Hit
AACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGG8140.10153765936984283No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT8130.10141292023056782No Hit
AGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCCGGACTCCCTAACGTT8080.10078922453419287No Hit
CTGTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCCG8080.10078922453419287No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATT8060.10053974625564288No Hit
TCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGT8060.10053974625564288No Hit
GCACTTGCACATGGGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTG8040.1002902679770929No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT37300.086.7067642
CTTCCGA38200.085.286631
CCGATCT41150.078.242034
CGATCTA7850.072.976735
TCCGATC53950.060.3506363
CGATCTG23600.058.9951325
CGATCTT7850.057.272885
CGATCTC14550.056.8157545
TCTAGCG902.0496373E-856.3863688
GATCTAA2650.054.7281886
GATCTCG6250.054.5311666
ATCTCTC1800.052.358777
TGCTCTT15200.050.569572
GATCTGA4950.049.8081746
ATCTCGC3500.049.7120637
ATCTAGC2500.049.29787
ATCTTGC3550.049.0118947
GTCTAGG604.9243204E-448.3734131
CGTAAGA1950.048.3432353
ATCTGAG1950.048.331177
Produced by FastQC (version 0.11.2)