FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2h8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2h8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209071
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3700.17697337268200752No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC3420.16358079312769347No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT3320.15879772900115272No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3260.15592789052522826No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3020.14444853662153048No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA3020.14444853662153048No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG2750.13153426347987046No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT2430.1162284582749401No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC2430.1162284582749401No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2320.11096708773574526No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA2130.10187926589531786No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA350.003559346862.1570056
CTTCCGA3100.056.1552471
TTCCGAT3200.054.387382
CGATCTG1750.049.7256055
ACTACCC450.00961989848.3443373
TGTAACC752.540183E-548.3443373
CTAATAT450.00961989848.3443373
CCGATCT3850.047.0886424
ACACATA951.9137042E-645.788944145
CTACACA657.315013E-444.625544
CATACTG700.001053541241.4380045
TCCCCCC2005.456968E-1239.874548
ATACTCC2200.039.5544624
GTATAAC750.001477305738.6847271
AATACAG750.001479044438.6754725
CCCCCCG2109.094947E-1237.9757549
CTAGTGG800.002030667636.258253
ACTCCCC2252.0008883E-1135.4525156
TATAAAG850.002733995734.1254165
ACAGCTA850.002737205234.1172528
Produced by FastQC (version 0.11.2)