FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2h7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2h7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213077
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC2960.13891691735851358No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG2500.11732847749874459No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC2400.1126353383987948No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA2280.10700357147885506No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA2180.10231043237890527No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT2170.10184111846891031No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG300.001933942872.497184
CTTCCGA4250.061.4382171
TTCCGAT4150.061.1422042
CCGATCT4300.059.009344
GTATAAG502.0023699E-458.0249861
ATCTAGA1203.59978E-948.3314552
TATGCGT450.00963028448.3314555
CGATCTA803.7255577E-545.310745
TACACCG657.324941E-444.613654
GATCTAG1401.400258E-841.446421
GTCGCGC700.001054969541.4269648
CGATCTG2650.041.036145
TATAGGT951.0264036E-438.156413
GGTCGCG850.002737688834.116327
TGCCGTG1707.77618E-834.11632145
TCCGATC7750.032.740663
ACTGAGC900.003622563632.220978
TACACCA900.003622563632.220975
TCGCGCG900.003622563632.220979
TTACCCC1402.7373517E-531.0702235
Produced by FastQC (version 0.11.2)