FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2h6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2h6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419310
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG10580.2523192864467816No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG7980.19031265650711884No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC5290.1261596432233908No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC5210.12425174691755503No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG4650.11089647277670459No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC4550.10851160239440985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT32450.067.024982
CTTCCGA32550.066.3973541
CCGATCT32700.065.1823044
TCCGATC34350.063.5286943
CGATCTG17050.062.5062035
ATCTAGC651.0926606E-555.7682237
CGATCTA6050.053.9246445
ATAGTGC858.9133937E-751.1755456
ATAGTAC450.00963595248.332463
AGGATAT604.9422163E-448.332466
GATCTGG9250.046.2424056
GATCTGA5500.043.499216
ATCTGGG4500.043.499217
GATGTAA907.460043E-540.2914621
GTGCTCT16800.040.1475681
CGATCTC12500.039.4392855
ATCTGAC3300.037.347817
TGCTCTT17200.037.092352
ATCTAAC1001.3902197E-436.2493487
TTATATA1001.3902197E-436.2493482
Produced by FastQC (version 0.11.2)