FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2h4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2h4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207653
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4930.23741530341483147No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG3860.1858870326939654No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA3700.1781818707170135No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3640.1752924349756565No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3570.17192142661074003No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3070.14784279543276524No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC2990.14399021444428925No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2870.13821134296157533No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG2810.13532190722021833No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC2690.1295430357375044No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG2610.12569045474902843No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG2380.11461428440716004No Hit
GTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATG2290.11028013079512455No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT2220.10690912243020809No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA353.4274617E-582.873661
TTCCGAT12250.062.74722
CCGATCT12500.061.4922564
CGATCTG5850.060.7385985
CTTCCGA13200.058.7806551
GTGTAAT400.00603939854.3858381
TCCGATC14500.053.0105673
TATATGC553.2098943E-452.7377783
GATCTAG553.2098943E-452.7377786
TGTTAAG1004.6717105E-850.7601132
GATCTGG2650.049.25516
TATAATA450.00962088548.3429685
CGATCTA2150.047.218715
GATCTCT1551.4551915E-1146.7835166
CGATCTC4750.044.2719845
CTGGGAG1557.3305273E-1042.1051644
TTAGTAG700.00105367241.436833
ATCTGGG1053.8105445E-641.4268537
ATCTCTC907.4637726E-540.2761047
CGATCTT2550.039.8118555
Produced by FastQC (version 0.11.2)