FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2h2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2h2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33930
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC670.197465369879163No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG590.1738874152667256No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT510.15030946065428824No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA490.1444149720011789No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC460.13557323902151489No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA410.12083701738874152No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA380.11199528440907752No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC350.10315355142941349No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT350.10315355142941349No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGCCT100.0070671183144.957239
GACTGGC100.0070671183144.957237
CCCCCCG203.8380685E-4108.717929
TCCCCCC203.8380685E-4108.717928
ACCATAC259.264464E-487.1028061
ACTCCCC300.001910596772.585676
ATACTCC350.003520307162.216294
CCATACT350.003520307162.216292
CTCCCCC350.003540997962.1245277
AAGACTG400.005972732354.4392555
CCAAGAG400.005972732354.4392553
ACTTGCC400.006007788254.358968
ATTCCTC400.006007788254.358968
CTTCCGA553.156248E-452.7895771
CCGATCT604.8489054E-448.3904464
TCTTCCG450.00951499548.3904465
TTCCGAT604.8489054E-448.3904462
CATACTC450.00951499548.3904463
ACCACCC450.00957076348.3190738
GTGCTCT700.001036188141.4775281