FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2h11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2h11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422202
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC7440.1762189662768059No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT6920.1639025869133732No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC6850.16224461276829574No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA6490.15371788859361157No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA6160.1459017247668178No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG5940.14069094888228856No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT5060.11984784534417176No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT4870.11534762980753288No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG4680.11084741427089403No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4470.1058734918356616No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA4440.10516293148777126No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC4230.10018900905253883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA9350.054.2967871
CGATCTG5400.053.7094965
CCGATCT10000.050.7554744
TTCCGAT10900.046.564652
CTAGGGG907.470336E-540.282123
CCCCCCG3550.038.8024029
TCCCCCC3550.038.8024028
GGGGGAT1051.850734E-434.527536
ACCATAC4350.033.3448261
CTCCCCC4150.033.1924177
GGGCGAC2807.2759576E-1231.0710987
GCCTATA950.004716273430.5368421
ACTCCCC4750.030.5296086
TACTCCC4550.030.277995
GTTCTAA1204.0459333E-430.2187481
ATACTCC4800.030.211594
CTAGCAC1204.0515544E-430.211593
TCGCGCG1703.006533E-629.8526259
TCCGATC17300.029.3384233
CCATACT4950.029.2960872
Produced by FastQC (version 0.11.2)