FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2g9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2g9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302622
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG31580.24243410598009246No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG29100.2233955821412505No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC27020.20742778795383465No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC25700.19729438010412845No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG23600.1811730494341413No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA21080.16182745263015672No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC20060.1539970920190201No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT19380.14877685161159568No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA15450.11860693278633402No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC14750.11323315589633831No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC13890.10663108714577216No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC13680.10501895407877343No Hit
GATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAG13650.10478864935491647No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT13530.10386743045948862No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG13330.10233206563377557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA90400.067.471731
TTCCGAT91650.066.377932
CCGATCT93600.064.9175954
CGATCTG51700.057.642865
TCCGATC113250.053.9738733
CGATCTA17750.052.696965
CGATCTC34350.045.384335
GATCTGA15500.044.909046
GATCTGG21850.041.4813426
ATCTGAC7700.040.486047
ATCTGTG5750.040.3468557
GTGTTAA19850.038.72931
TGTTAAG19750.038.5492972
GATCTAG8000.038.067436
GTGCTCT47250.037.4526251
ATCTCGC7250.036.9991077
GATCTCA12550.036.9768946
TGCTCTT48350.036.592122
GTTAAGA21450.036.5082243
GTCTAGG1001.3887377E-436.2630461
Produced by FastQC (version 0.11.2)