FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2g8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2g8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382756
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC7810.20404644211978387No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG6560.1713885608586149No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC5910.154406462602807No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5900.15414519955271766No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA5820.15205509515200283No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5790.15127130600173477No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4500.11756837254020837No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC4410.11521700508940422No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT4350.11364942678886811No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT4310.11260437458851069No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG4220.11025300713770653No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT4120.107640376636813No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT3990.10424395698565142No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA6700.055.2140271
CCGATCT7000.053.8628234
TTCCGAT7400.051.9311562
CGATCTG4100.049.5174145
GTCTAGG752.5401248E-548.357381
CTGTGCG450.00963558748.332117
CCTACAT1109.9294994E-846.1412283
TCCCCCC2950.044.236178
CCCCCCG3200.040.780229
TACCTAG750.001481473638.6707422
CTCCCCC3400.038.3813827
GTATTAA951.0246952E-438.176881
ACTCCCC3450.037.830086
GGGCGAC2151.2732926E-1137.0920877
CTACATT1854.1636667E-935.273994
TCGCGCG1854.1691237E-935.2693829
TCTTCCG4400.034.6059235
TCTATAC1905.4023985E-934.3457263
CCCAGAC1301.6509326E-533.4650652
AGGTCGC1956.9612724E-933.4650656
Produced by FastQC (version 0.11.2)