FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2g6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2g6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391450
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG7790.19900370417677862No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG7510.1918508110869843No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5370.13718227104355601No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC4790.122365563928982No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC4490.11470174990420232No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT4170.10652701494443734No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC4170.10652701494443734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT30900.060.3048742
CTTCCGA31200.059.965081
CCGATCT31200.059.0203064
TCATACA502.0090854E-457.9979024
CGATCTA5300.056.0828825
TCCGATC35050.053.157853
CGATCTG16700.048.6215
CGATCTC11350.045.989535
TATCGTG1201.9698928E-742.290145
ATCTGAG1401.411172E-841.4270747
GATCTAA1601.0077201E-940.7797786
GATCTGG6250.040.5985346
GATCTCT3050.040.4083756
TATAGAA951.0271901E-438.1613922
ATAGCAC951.02796315E-438.1565173
GATCTGA5700.038.1565136
ATAGGGT1001.3901987E-436.2486884
GATCTAC3250.035.691026
ATCTGTG2252.0008883E-1135.443167
TCTGTGC2052.910383E-1035.3645748