FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2g5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2g5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396266
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG10400.2624499704743783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT32200.055.3866542
CTTCCGA32450.054.7434431
CGATCTG15350.052.8975525
CCGATCT34800.051.0402454
TCCGATC35200.050.4602433
GATCTAA1950.044.6141436
GTATAGA1652.7284841E-1143.9437141
CGATCTA6900.042.0278175
CTCTAGG750.001481588138.670471
GATCTGC9450.037.5915456
GATCTGA5550.036.5755586
TCTAGCC1251.263406E-534.799033
CGATCTC14300.033.460615
GTTATAT900.003623863232.225391
ATTACAT1352.1393662E-532.2213253
ATATGAG900.003626103732.2213255
TTACATA900.003626103732.2213254
ATCTGAC2257.894414E-1032.2213257
TTATACT1153.1591966E-431.520864
GCTAGGG1153.1591966E-431.520862
Produced by FastQC (version 0.11.2)