FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2g3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2g3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209943
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG3730.17766727159276566No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3210.15289864391763477No Hit
GTACAGGAATATTCACCTGTTGTCCATCGACTACGCCTTTCGGCCTGATC2840.1352748126872532No Hit
GTGATAAAGGAGGTAGGGGTGCAGAGACAGCCAGGAGGTTTGCCTAGAAG2650.12622473719057078No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC2370.11288778382703878No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGGA203.862741E-4108.7430343
CATTAGG451.2299515E-680.55042
CCGATCT12800.073.62814
TAGGACA300.001934096272.495354
CTTCCGA13250.072.2217941
TTCCGAT13400.071.413342
GATCTAG754.833055E-967.662336
ATCTAGC451.1920479E-464.4403157
TCCGATC15150.063.6427843
TCTAGAC350.003563484462.1388743
CGATCTA1550.060.802565
CGATCTC5000.056.546375
GTCTAGG400.006045786754.3715171
TGCTCTT5700.050.8739282
CGATCTG9450.050.6316765
GATCTCG2600.050.189096
ATGTACA450.00963104848.330244
ATATAGG450.00963104848.330242
ATCTTAC604.9376563E-448.3302357
TCTGGTC1056.875052E-848.3302358
Produced by FastQC (version 0.11.2)