FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2g1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2g1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392212
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG9820.25037479730349915No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC5860.14940899309557076No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC5280.13462107227723782No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA4970.1267171835639909No Hit
ATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGCGCGAACTTC4300.10963458537729595No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4190.1068299797048535No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG4150.1058101230966926No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC4000.10198566081608924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT23800.067.92932
CTTCCGA24500.066.022131
ATCTAAC554.057494E-665.907757
CCGATCT24600.065.13084
TCCGATC27350.059.1121523
CGATCTG14450.055.1891945
ATATTAC553.208815E-452.753111
TACTAGT450.009635569548.332364
GATCTAA1056.893242E-848.332366
CTAGACA752.5480282E-548.3323554
CGATCTA4500.046.721285
GATCTGG6900.044.1295436
CGATCTC7600.043.880695
CGATCTT6000.043.4991235
GTGCTCT12500.042.941031
TCTGACC1401.4109901E-841.4277348
TGCTCTT13150.041.3489722
CTATAAT907.452555E-540.2975121
ATCTTAC1303.6912934E-739.037677
TCTAGAC1157.1326085E-637.8253253