FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2g12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2g12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038355
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG30090.2897852853792778No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC21540.20744350438915402No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG21470.20676936115297753No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC19770.19039731113154942No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC19350.18635245171449072No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC15150.1459038575439036No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC15120.14561493901411368No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA14790.1424368351864247No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA14130.13608062753104672No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG13730.13222838046718124No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT12750.12279037516071092No Hit
ATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGCGCGAACTTC12620.12153839486495467No Hit
ACCTCCGGCTGAGCCGTTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGA11830.11393020691382041No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT11110.10699616219886263No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG11080.10670724366907271No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT67450.069.439832
CTTCCGA68700.068.506171
CCGATCT69500.067.388364
CGATCTG33300.062.4848565
TCCGATC79450.059.313923
CGATCTA13950.057.6880575
CGATCTC25500.047.195985
GATCTGA10600.047.1933026
CGATCTT13500.045.648065
GATCTGG16050.041.557556
ATCTGAC5850.040.8973547
CTCTACT4450.040.7400631
TCTACTT4950.036.617852
CGCTAGG1001.3895328E-436.2586561
GATCTAA4400.036.2499286
GTGCTCT35400.036.0538061
GTGTTAA8750.035.637081
CTACTTG5300.035.5659683
TATGCTA1051.8537868E-434.5237435
TGCTCTT37150.034.1536642
Produced by FastQC (version 0.11.2)