FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2g11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2g11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674548
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC11830.1753766966917106No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG10760.15951422285738004No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA9620.14261401708996246No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC9510.14098329548082567No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT8840.13105071840699253No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA8840.13105071840699253No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT7870.11667071876278634No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC6980.10347669847067963No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT6940.10288370879462988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA12000.058.6250571
TTCCGAT12350.056.3680042
CCGATCT12550.054.8796924
TCCCCCC4950.049.7972688
CCCCCCG5100.046.911099
ACTCCCC5950.042.6464466
CTCCCCC6050.040.743227
TGCCTAC1901.2914825E-1038.1714971
CGATCTG8250.037.787345
TTAGGGG1354.965859E-737.594843
CCATACT7400.037.237482
GTACAGG1209.535901E-636.262921
ATACTCC7000.036.249484
TAGGGGG1001.3910866E-436.2494774
GGTCGCG2900.034.99957
GTCGCGC2950.034.4062848
ACCATAC7800.034.4032821
GGGCGAC4900.034.0301257
TCTTCCG8300.033.19235
TCCGATC21050.032.7216723