FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608242
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG19890.3270080001052213No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6450.1060433182844986No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT42750.062.942292
CCGATCT42400.062.9331634
CTTCCGA43300.062.320541
CGATCTG20600.057.7263685
TCCGATC47850.056.522913
CAGTATA651.0933085E-555.767719
CGATCTC15900.049.708085
GATCTAG2850.045.7957576
CGATCTA7400.040.174435
GATCTGG11300.039.142546
ATCTGTG3350.038.9541667
TATACAG1502.5935151E-838.6719745
CGATCTT10600.036.939035
TGTTAAG12900.036.5450362
GTGTTAA12950.036.4099241
TTATATA1001.3876702E-436.263922
GATCTTA2002.237357E-1036.2549746
CTATACA1656.002847E-835.1563384
TACAGTA1251.2645103E-534.7990537
GTTAAGA14150.033.8208923
Produced by FastQC (version 0.11.2)