FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254452
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG3840.15091254932167952No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT3700.14541052929432663No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG3600.14148051498907455No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC3330.13086947636489396No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3260.1281184663512175No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC3230.1269394620596419No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGAC3030.11907943344913775No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC2930.11514941914388568No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC2830.11121940483863361No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG2780.10925439768600757No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAA2770.10886139625548236No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT2740.10768239196390676No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGC809.822543E-1172.49987
TTCCGAT16850.060.667492
CCGATCT17200.059.0114674
CTTCCGA17550.057.8573381
CGATCTG7150.056.7830625
TCCGATC18700.054.2779273
CTTTATA553.2144785E-452.727132
GATCTCT1402.7102942E-1046.6070186
CGATCTA3800.045.789355
CTAGGGG951.9152394E-645.789353
CGATCTC5950.045.0839165
TACACCG700.001055169341.428465
GATCTAG1859.640644E-1139.1890876
TACACTT750.001481326438.666565
TCTAGCC951.0267481E-438.157798
CGATCTT4600.037.8259855
TACTGGA800.002033794836.24997
TGGGGCA800.002033794836.24995
GATCTGG3850.035.779126
ATCTGGA1458.664756E-734.9999052