FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496485
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG9640.1941649798080506No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5590.11259151837417043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA29750.063.1289141
TTCCGAT31250.059.622632
CCGATCT31400.058.8760384
TCCGATC34300.054.5322763
CGATCTA5950.048.738385
CGATCTG16700.048.621645
CTAGACA604.943217E-448.332234
GATCTAG2400.048.332236
ATCTAGC1801.8189894E-1244.304547
ATCTAGA1151.4109173E-744.129422
CTGTAAG907.469125E-540.284971
TATTAGG907.478001E-540.2768552
TTAGGTG1451.9248546E-839.999084
TAATACT1451.9248546E-839.999084
TCTAGAC1105.2597716E-639.5445483
CATATTA750.001482833438.665782
TCAGGGG1901.2914825E-1038.157023
CGATCTC14250.037.13955
ATCTGTG2350.037.020437
ATTCTAA1001.3889806E-436.256471
Produced by FastQC (version 0.11.2)