FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374460
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG5910.1578272712706297No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4430.11830369064786626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT24100.061.98422
CGATCTG12550.061.2399675
CTTCCGA24700.059.613681
CCGATCT25400.057.3766444
TCCGATC27700.053.397783
CGATCTA5300.045.1451265
GATCTGG6200.044.4390456
TATATGT1201.9677827E-742.2950554
GATCTAG2150.040.468366
ATACTAA907.4523065E-540.297151
TCTCGTT2900.037.518036145
TTATATG1209.542637E-636.2529033
TACACCG1604.573303E-836.2529035
ATCTGGG3000.036.2480627
GATCTCA3450.035.7274976
ATCTGGC3700.035.2683877
CGATCTC10050.034.6296425
TAGATAG1051.8506357E-434.5265775
TACAGTG850.002738729834.1203775
CTATGGG900.003621563332.2291072
Produced by FastQC (version 0.11.2)